pgp - publications

Predict more pgp - ligand interactions now!


1. Cytometry A. 2012 Mar 7. doi: 10.1002/cyto.a.22038. [Epub ahead of print]

DyeCycle violet used for side population detection is a substrate of
P-glycoprotein.

Boesch M, Reimer D, Rumpold H, Zeimet AG, Sopper S, Wolf D.

Internal Medicine V, Tyrolean Cancer Research Institute, Innsbruck Medical
University, Innsbruck, Austria.

Cancer stem cells (CSC) are increasingly recognized as key target cells for
cancer therapy because they are both tumorigenic and chemoresistant and,
therefore, can give rise to recurrent disease. Side population (SP) sorting using
an ultraviolet (UV) laser is an established method to isolate CSC based on ABC
drug transporter-dependent (e.g., ABCG2) Hoechst 33342 efflux. We here show that
Vybrant® DyeCycle™ Violet (DCV), a DNA-binding fluorophor allowing SP sorting
using a non-UV laser (such as violet laser), is transported via P-glycoprotein
(PgP). Because PgP might be particularly abundant in multidrug-resistant cancer
cells rather than bona fide CSC, investigators using DCV should be aware that
this strategy might also detect PgP-expressing non-CSC. © 2012 International
Society for Advancement of Cytometry.

Copyright © 2012 International Society for Advancement of Cytometry.

PMID: 22407964 [PubMed - as supplied by publisher]