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[Effect of Shaoyao Gancao Tang on function and expression of P-glycoprotein in Caco-2 cells].


Zhongguo Zhong Yao Za Zhi. 2012 Apr;37(7):991-6


Authors: Wang Y, Zhao J, Zhao Y, Li C, Yi Y, Liang A, Odd GN


Abstract

OBJECTIVE: To investigate the effect of Shao Yao-Gan Cao-Tang on function and expression of P-glycoprotein (P-gp) in Caco-2 cells.

METHOD: 3H-digoxin (Dig), a substrate of P-glycoprotein, was used as a probe to measure the P-gp-mediated drug efflux transport, which indicated the function of P-gp in Caco-2 cells, while Verapamil (Ver) was used as a positive P-gp inhibitor. P-gp expression in Caco-2 cells was tested by immunohistochemistry staining. Inhibition effect of SGT on P-gp-mediated drug efflux transport and P-gp expression were investigated.

RESULT: Dig was shown a positive absorption mode in Caco-2 cell monolayer, characterized as the ratio of apparent permeabilities (Papp) from basolateral side to apical side Papp (BL-->AP) and from apical side to basolateral side Papp (AP-->BL) of Dig was 27.07. Addition of Ver into Dig transport media significantly inhibited P-gp activity which was indicated by increasing the Papp (AP-->BL) of Dig by 3.82 times, whereas Ver had no significant effect on Papp (BL-->AP). SGT (at the concentrations of 1/25 IC5, 1/5 IC5, IC,) could promote Papp (AP-->BL) of Dig by 159.83%, 217.95% ,160.26%. Papp (AP-->BL) of Dig was mildly increased by 59.16%, 50.73% by SGT at 1/25 IC5, 1/5 IC, respectively. Immunohistochemistry staining showed that SGT inhibited the expression of P-gp of Caco-2 cells.

CONCLUSION: SGT showed the potential inhibition to the function and expression of P-gp, leading to the increase absorption of P-gp's substrates.

PMID: 22792804 [PubMed - in process]