pgp - publications

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1. Zhonghua Gan Zang Bing Za Zhi. 2011 Oct;19(10):755-8. doi:

[Expressions and significance of COX-2 and P-gp in human hepatocellular cacinoma

[Article in Chinese]

Li B, Liu Y, Su S, Zhang MY, Yuan Q, Chen C, Xia XM, Liu CA, Gong JP.

Department of Hepatobiliary Surgery, The Affiliated Hospital of Luzhou Medical
College, Luzhou 646000, China.

OBJECTIVE: To investigate the effect of the COX-2 gene on the oncogenesis and
development of the hepatocellular cacinoma and the influence of COX-2 gene on the
expression of P-gp protein.
METHOD: Fifty-two pieces of the hepatocellular cacinoma samples and 20 cases of
normal liver samples were collected from the patients operated from October 2003
to June 2005. RT-PCR and immunohistochemistry staining were employed to detect
the COX-2 mRNA as well as P-gp protein in the normal liver tissues and the
carcinoma tissues. Meanwhile, the expression of the mdr 1 mRNA in the carcinoma
tissues was also determined and the correlation between the expressions of the
COX-2 and P-gp was investigated.
RESULTS: No expression of the COX-2 in the normal liver tissue was detected. The
positive expression of COX-2 in the low and middle differentiated carcinoma was
elevated significantly as compared with that in the high differentiated carcinoma
tissue (x2 = 6.80, P less than 0.01). The positive expression of the COX-2 in the
HBSAg (+) carcinoma tissue was significantly higher as compared with that in the
HBSAg (-) carcinoma (x2 = 4.70, P less than 0.05), and the carcinoma in
combination with cirrhosis also showed significantly higher in expression of
COX-2 than the carcinoma without cirrhosis (x2 = 7.51, P less than 0.01). The
mdr1 mRNA was found both expressed in the normal and carcinoma tissues. The
expression of COX-2 mRNA was found in the carcinoma, but not found in the normal
tissues. The COX-2 mRNA and mdr1 mRNA was found both expressed in the normal and
carcinoma tissues. The correlation coefficient between COX-2 and mdr1 mRNA was
0.563 ( P less than 0.01).
CONCLUSION: The results indicated that Cox-2 gene might involved in the multidrug
resistance of the hepatocellular carcinoma mediated by P-gp.

PMID: 22409848 [PubMed - in process]