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1. Oncol Rep. 2012 May;27(5):1567-72. doi: 10.3892/or.2012.1697. Epub 2012 Feb 22.

Inhibitory effects of epigallocatechin-3-gallate on cell proliferation and the
expression of HIF-1α and P-gp in the human pancreatic carcinoma cell line PANC-1.

Zhu Z, Wang Y, Liu Z, Wang F, Zhao Q.

Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong
University of Science and Technology, Wuhan 430030, P.R. China.

The aim of this study was to verify the inhibitory effects of
epigallocatechin-3-gallate (EGCG) on cell proliferation and the expression of
hypoxia-inducible factor 1 (HIF-1α) and multidrug resistance protein 1
(MDR1/P-gp) in the human pancreatic carcinoma cell line PANC-1, thereby,
reversing drug resistance of pancreatic carcinoma and improving its sensitivity
to cancer chemotherapy. The human pancreatic carcinoma cell line PANC-1 was
incubated under hypoxic conditions with different concentrations of
epigallocatechin-3-gallate (EGCG) for indicated hours. The effects of EGCG on the
mRNA or protein expression of HIF-1α and MDR1 were determined by RT-PCR or
western blotting. Cellular proliferation and viability assays were measured using
Cell Counting Kit-8. Western blotting revealed that EGCG inhibits the expression
of the HIF-1α protein in a dose-dependent manner, while RT-PCR showed that it
does not have any effects on HIF-1α mRNA. In addition, EGCG attenuated the mRNA
and protein levels of P-gp in a dose-dependent manner, reaching a peak at the
highest concentration. Furthermore, EGCG inhibited the proliferation of PANC-1
cells in a concentration- and time-dependent manner. The attenuation of HIF-1α
and the consequently reduced P-gp could contribute to the inhibitory effects of
EGCG on the proliferation of PANC-1 cells.

PMID: 22367292 [PubMed - in process]