pgp - publications

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1. Cancer Genomics Proteomics. 2012 May;9(3):153-61.

Modulation of Apoptosis Protein Profiles - Role of P-gp in HeLa Cells Exposed to
Doxorubicin.

Gruber BM, Krzyszton-Russjan J, Bubko I, Koronkiewicz M, Anuszewska EL.

Department of Biochemistry and Biopharmaceuticals, National Medicines Institute,
Chełmska 30/34 Str., 00-725 Warsaw, Poland. b-gruber@il.waw.pl.

As shown previously doxorubicin (1 μM) plus sulindac (50 μM) reduced the
expression of ABCB1 (ATP-binding cassette, sub-family B (MDR/TAP), member 1) mRNA
in HeLa cells and this effect was accompanied by increased apoptosis. The aim of
this study was to define if the decrease of ABCB1 expression or blocking of
P-glycoprotein (P-gp) can affect the expression of the apoptotic genes determined
with use of quantitative real time polymerase chain reaction (qRT-PCR). Western
blot was used for visualization of chosen pro- and antiapoptotic proteins.
Doxorubicin was the main compound which affected the apoptotic genes. The
effectiveness of the drugs in reducing of P-gp function has been shown as not
being related to the regulation of apoptotic gene transcription. In this
experimental scheme, regulation of apoptotic gene transcription depended on the
kind of P-gp modulator.

PMID: 22593250 [PubMed - in process]