pgp - publications

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1. Macromol Biosci. 2012 Jan 25. doi: 10.1002/mabi.201100350. [Epub ahead of print]

Targeting of Multidrug-Resistant Human Ovarian Carcinoma Cells With
Anti-P-Glycoprotein Antibody Conjugates.

Fowers KD, Kopeček J.

Department of Bioengineering, University of Utah, Salt Lake City, UT 84112, USA.

A monoclonal antibody (mAb) to P-glycoprotein (Pgp), UIC2, is used as a targeting
moiety for N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer/drug [(meso chlorin
e(6) mono(N-2-aminoethylamide) (Mce(6) ) or doxorubicin (DOX)] conjugates to
investigate their cytotoxicity towards the Pgp-expressing human ovarian carcinoma
cell line A2780/AD. The binding, internalization, and subcellular trafficking of
a fluorescein labeled UIC2 targeted HPMA copolymer are studied and show
localization to the plasma membrane with limited internalization. The specificity
of the UIC2-targeted HPMA copolymer/drug conjugates are confirmed using the
sensitive cell line A2780 that does not express Pgp.

Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PMID: 22278817 [PubMed - as supplied by publisher]